Tris:(Tris(Hydroxymethyl)
Aminomethane)
Catalog # Size
IB70142 500 g
IB70144 1 kg
Physical Specifications
Molecular formula C4H11NO3
Molecular weight 121.14
Purity >99.8%
Lead
<0.0001%
Moisture <1.0%
Iron
<0.0001%
Insolubles <0.005%
Copper
<0.0001%
A280 (1M, water) <0.05
Magnesium
<0.0001%
Arsenic <0.0005%
Molecular Biology Specifications
DNase assay
None Detected
RNase assay
None Detected
Protease assay None
Detected
Recommended Use
IBI Molecular Biology Grade Tris is commonly used in biological and electrophoresis buffer
systems. Located in the table below are some of the commonly used electrophoresis buffers
and their recipe as a concentrated stock.
Buffer
Concentrated stock
Solution (per liter)
Tris- acetate (TAE buffer)
50X:
242 g Tris base
57.1 mL glacial acetic acid
100 mL 0.5 M EDTA (pH 8.0)
Tris- borate (TBE buffer)
5X:
54 g Tris base
27.5 g boric acid
20 mL 0.5 m EDTA (pH 8.0)
Tris- phosphate (TPE buffer)
10X:
108 g Tris base
15.5 mL 85% phosphoric acid
(1.679 g/ mL)
40 mL 0.5 M EDTA (pH 8.0)
Tris- glycine
5X: 15.1 g Tris base
94 g glycine (pH 8.3)
50 mL 10% SDS
Storage
IBI's should be stored at room temperature and protected from moisture.
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