0.5 M EDTA Solution, pH 8
Catalog # Size
IB70184 100 mL
IB70185 4 X 100 mL
Physical Specifications
Purity(EDTA component; dry basis) >99.0%
Insolubles
<0.01%
Heavy Metals
<0.005%
A280-600
<0.10
Molecular Biology Specifications
DNase assay None
Detected
RNase assay None
Detected
Protease assay None
Detected
Recommended Use
0.5 M EDTA is commonly used as a component in buffers. Located in the table below are some
of the commonly used electrophoresis buffers and their recipe as a concentrated stock.
Buffer
Concentrated stock solution (per liter)
Tris-acetate (TAE buffer) 50X:
242 g Tris base
57.1 mL glacial acetic acid
100 mL 0.5 M EDTA (pH 8.0)
Tris-borate (TBE buffer) 5X:
54 g Tris base
27.5g boric acid
20 mL 0.5 M EDTA (pH 8.0)
Tris-phosphate (TPE buffer) 10x:
108 g Tris base
15.5 mL 85% phosphoric acid
(1.679 g/Ml)
40 mL 0.5M EDTA (pH 8.0)
Alkaline Buffer 1X:
5 mL
10N NaOH
2 mL 0.5 M EDTA (pH 8.0)
Storage
Store at room temperature. Protect from moisture.
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