EDTA, disodium salt (ethylenediaminetetracetic acid,
disodium salt)
Catalog # Size
IB70180 100 g
IB70182 500 g
Physical Specifications
Formula Weight 372.24
Molecular Formula C10H14N2O8Na2 .2H2O
Purity
>99.0%
Insolubles <0.01%
Heavy Metals <0.005%
A330-600 (0.5M, water) <0.10%
Molecular Biology Specifications
DNase assay None
Detected
RNase assay None
Detected
Protease assay None
Detected
Recommended Use
EDTA is commonly used in biological and electrophoresis buffer systems. Located in the
table below are some of the commonly used electrophoresis buffers and their recipe as a
concentrated stock.
Buffer
Concentrated stock solution (per liter)
Tris-acetare (TAE buffer) 50X:
242 g Tris base
57.1 mL glacial acetic acid
100 mL 0.5 M EDTA (pH 8.0)
Tris-borate (TBE buffer) 5X:
54 g Tris base
27.5g boric acid
20 mL 0.5 M EDTA (pH 8.0)
Tris-phosphate (TPE buffer) 10x:
108 g Tris base
15.5 mL 85% phosphoric acid
(1.679 g/Ml)
40 mL 0.5M EDTA (pH 8.0)
Sambrooks, J., Fritsch, E.F., Manlatis, T (1989) Molecular Cloning A Laboratory Manual
vol. 3 8.23
Storage
Store at room temperature. Protect from moisture.
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