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Dithiothreitol (DTT, Cleland's)

Catalog #    Size
IB21040        5 g


Physical Specifications
   
Formula Weight        323.13
Molecular Formula        C11H12Cl2N3O5
-SH content              >99.4%
A283 90.02 M, water)        <0.05
-S-S-content            <0.5%
Melting Point            41 ± 2C
IR                 Passes
Moisture            <0.5%
Solubility (5%, water)        Clear and Colorless

Molecular Biology Specifications
Antibiotic Inhibition/Sensitivity Assay        Pass


Recommended Use
Chloramphenicol is used in the amplification of plasmids and colony hybridizations.

Stock Solution                     Working Concentration
Concentration                         stringent     relaxed
                            Plasmids     plasmids

Chloramphenicol    34 mg/mL in ethanol    25 ug/ul     170ug/mL
Note: Antibiotics dissolved in ethanol need not be sterilized

Storage
Store at +4°C. Warm to room temperature before opening. Keep tightly sealed. Store Chloramphenicol solution in a light-tight container at -20°C