Acrylamide
Catalog # Size
IB70020 100 g
IB70024 500 g
IB70026 1.5 kg
IB70028 3 kg
Physical Specifications
Formula Weight 71.08
Molecular Formula C3H5NO
Purity
>99.9% Conductivity
<5 mmho
Melting Point 85 ± 1°C
Iron
<0.0001%
pH (10%, 0.1 M NaCL) 6.5 ± 0.5
Lead
<0.0001%
Acrylic Acid <0.001%
A290 (1%,water)
<0.15
Molecular Biology Specifications
Dnase assay None Detected
Polymerization assay
Pass
Rnase assay None Detected
Gel analysis
Pass
Protease assay None Detected
Recommended Use
A primary component of polyacrylamide gels for sequencing and protein electrophoresis.
Recipe for a 6% 100ml Sequencing gel:
5.7 g Acrylamide
0.3 g Bisacrylamide
48 g Urea
10 ml 10X TBE buffer (if using a Klenow or
reverse Transcriptase kit)
or
5 ml 20X modified TBE buffer (if using Taq
polymerase kit)
1.0 ml 10% Ammonium persulfate (APS)
50 ml TEMED
Storage
Store at +4°C. Keep tightly sealed. Protect from moisture.
Warning
Poison/Neurotoxin/Lachrymator. Suspected carcinogen. May be harmful or fatal if swallowed.
Avoid breathing dust. Avoid contact with eyes, skin, and clothing. Wash thoroughly with
water after handling. See Material Safety Data Sheet for additional information.
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