Methylene Blue, Chloride, Trihydrate
Catalog # Size
IB74050 25 g
Physical Specifications
Formula Weight 373.9
Molecular Formula
C16H18CIN3S . 3H2O
Em (661 nm, water)
>69,000
Ash
<1.0%
Arsenic
0.0008%
Copper
<0.01%
Moisture
<15.0%
Zinc
<0.001%
Lead
<0.001%
Molecular Biology Specifications
DNase assay
None Detected
RNase
None Detected
Protease assay
None Detected
Gel Analysis assay Pass
Recommended Use
Methylene blue is a recommended stain for agarose gels when recovery of DNA is required.
The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a
white light box for visualization and photography instead of a UV light box. Using a white
light box eliminates mutations that may affect the integrity of the isolated DNA.
1. Stain a 1% gel for 15 minutes with 0.02% Methylene Blue Solution in distilled water.
2. Destain with distilled water for 15 minutes.
Used for staining RNA that is bond to nylon and nitrocellulose membranes.
1. Soak the dried filter in 5% acetic acid at room temperature for 15minutes.
2. Place the filter in 0.5 M Sodium acetate (pH 5.2) and 0.04% methylene blue for 5-10
minutes at room temperature.
3. Rinse the filter in water for 5-10 minutes.
Storage
Store at room temperature. Protect from moisture.
Warning
Avoid contact with eyes, skin and clothing. Do not take internally. Wash thoroughly with
water after handling. See Material Safety Data Sheet for additional information.
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