Ethidium Bromide Solution
(10 mg/ml)
Catalog # Size
IB40075 10 mL
Physical Specifications
Purity
>98.0%
Melting Point 272.5 ±
2.5oC
Moisture <8.0%
Ammonium Bromide <0.1%
Molecular Biology Specifications
DNase assay
None Detected
RNase assay
None Detected
Recommended Use
Ethidium Bromide can be used to detect both single-and-double stranded nucleic acids (both
DNA and RNA). However, Ethidium Bromide does not intercollate into single-stranded DNA
very well, therefore, the fluorescence of single-stranded DNA is relatively poor in
comparison to double-stranded DNA. The recommended final concentration of Ethidium Bromide
in agarose gels and electrophoresis buffers is 0.5 ug/mL.
Storage
Store at room temperature.
Protect from moisture.
Warning
Poison/Suspected mutagen. Avoid contact with eyes, skin and clothing. Avoid breathing
dust. Do not take internally. Wash thoroughly with water after handling. See Material
Safety Data Sheet for additional information.
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