Ethidium Bromide
Catalog # Size
IB40060 5 grams
Physical Specifications
Acrylamide Component
Formula Weight 394.32
Molecular Formula C21H20BrN3
Purity
>98.0%
Melting Point 272.5 ±
2.5°C
Moisture <8.0%
Ammonium Bromide <0.1%
Molecular Biology Specifications
DNase assay
None Detected
RNase assay
None Detected
Recommended Use
Ethidium Bromide can be used to detect both single-and-double stranded nucleic acids (both
DNA and RNA). However, Ethidium Bromide does not intercollate into single-stranded DNA
very well, therefore, the fluorescence of single-stranded DNA is relatively poor in
comparison to double-stranded DNA. The Recommended final concentration of Ethidium Bromide
in agarose gels and electrophoresis buffers is 0.5 ug/mL.
Storage
Store in the dark at room temperature, protected from moisture. Keep tightly sealed.
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