Agarose, Low Melting Point
Catalog # Size
IB70050 50 g
Physical Specifications
Melting Point <65oC
EEO (-mr): <0.15
Sulfate:
<0.15%
Gel Strength: >200
g/cm²
Gelling Point (dynamic): 28 ± 2°C
Molecular Biology Specifications
DNase assay
None Detected
RNase assay
None Detected
Protease assay
None Detected
Gel analysis assay Pass
DNA Extraction assay
Pass
Recommended Use
IBI Molecular biology Grade LMP agarose is commonly used as a gel matrix when fragment
recovery after electrophoresis is desired. It can also be used for preparing large DNA
samples for plugs.
To prepare a gel: Add the correct amount of LMP agarose to
electrophoresis buffer (for example TAE or TBE) in beaker or flask. The buffer should not
occupy more than 50% of the volume of the flask or bottle. Heat the LMP agarose slurry in
a microwave oven. After 90 seconds in the microwave, swirl the flask to make sure any
grains sticking to the walls enter into the solution. Undissolved LMP appears as small
"lenses" floating in the solution. Repeat heating until the agarose dissolves
completely. If the volume of the solution has decreased due to evaporation; replenish with
water.
If you desire to add ethidium bromide to the solution, allow the solution to cool to
approximately 60°C. Then add ethidium bromide to a final concentration of 0.5 mg/mL and
mix thoroughly. Pour into a casting tray and insert comb, allow the gel to harden for 30
minutes. Load sample and electrophorese according to your sample specifications and
percentage gel.
(It is important to use the same lot of electrophoresis buffer in both the buffer tanks
and in the gel. Small differences in ionic strength or pH create fronts in the gel that
can greatly affect the mobility of the fragments.)
Amount of agarose in gel Efficient range of separation
(% [w/v]) of linear DNA molecules (kb)
0.3 % 5-60 kb
0.6 % 1-20 kb
0.7 % 0.8-10 kb
0.9 % 0.5 - 7 kb
1.2 % 0.4 - 6 kb
1.5 % 0.2 - 3 kb
2.0 % 0.1 - 2 kb
Sambrook, J., Fritsch, E.F., & Maniath, T. (1989) Molecular
Cloning, A Laboratory Manual, 1,6.8-6.13.
Storage
IBI LMP agarose should be stored at room temperature protected from excessive moisture.
LMP agarose gels may be stored wrapped in plastic or submerged in 1X running buffer at
+4°C until |
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