Acrylamide:Bisacrylamide 19:1
Catalog # Size
IB70022 40 g
IB70023 200 g
Physical Specifications
Acrylamide Component
Formula Weight 71.08
Molecular Formula C3H5NO
Purity
>99.9% Conductivity
<5 mmho
Melting Point 85 ± 1°C
Iron
<0.0001%
pH (10%, 0.1 M NaCL) 6.5 ± 0.5
Lead
<0.0001%
Acrylic Acid <0.001%
A290 (1%,water)
<0.15
Bisacrylamide Component
Formula Weight 154.17
Molecular Formula C7H10O2N2
Purity
>99.0% Conductivity
<10 mmho
A290 (2%, water) <0.4
Acrylic Acid
<0.001%
Molecular Biology Specifications
DNase assay
None Detected Polymerization assay
Pass
RNase assay
None Detected Gel analysis
Pass
Protease assay
None Detected
Recommended Use
A primary component of polyacrylamide gels used for sequencing. A sequencing gel is a
high-resolution denaturing polyacrylamide gel (typically 6% or 8%) designed to fractionate
radiolabeled single-stranded oligodeoxynucleotides on the basis of size.
Recipe for a 6%, 100mL gel:
Add: 6 g acrylamide:bisacrylamide, 19:1 (or 15 mL of 40% solution), 48 g
Urea, 10 mL of 10X TBE buffer
(if using a Klenow or Reverse Transcriptase kit) or 5 mL of 20X modified TBE buffer (if
using a TaqPolymerase kit). Bring to a final volume of 99 mL with water. Mix with a stir
bar until completely dissolved. Filter sterilize.
Add: 1.0 mL of 10% Ammonium persulfate (APS) and 50mL TEMED
Pour gel immediately. Allow to polymerize for 1 hour.
To make a 40% solution:
For the 40 g size, add 63 mL double distilled H2O directly to the
bottle.
For the 200 g size, add 325 mL double distilled H2O directly to the
bottle.
Storage
Store at +4°C. Keep tightly sealed. Protect from moisture.
Warning
Poison/Neurotoxin/Lachrymator. Suspected carcinogen. May be harmful or fatal if swallowed.
Avoid breading dust. Avoid contact with eyes, skin, and clothing. Wash thoroughly with
water after handling. See Material Safety Data Sheet for additional information.
|
|