Acridine Orange
Catalog # Size
IB74020 5 grams
Physical Specifications
Formula Weight 369.94
Molecular Formula
C17H20ClN3
Purity
>98.0%
Melting Point
105 ± 1° C
Moisture
<1.6%
Solubility (1% Solution) Complete
Molecular Biology Specifications
DNase assay
None Detected
RNase assay
None Detected
Recommended Use
Acridine Orange is a metochromatic stain that can be used to detect both single-and
double-stranded nucleic acids. Acridine orange interacts differently with polynucleotides,
either by intercalation between the stacked basses of dsDNA or RNA or when the
polynucleotide is predominantly single-stranded, by binding electrostatically to the
phosphate backbone to produce a stacked array. If the stain intercalates between the
stacked basses of the double stranded nucleic acid it will fluoresce green at 530 nm. If
acridine orange binds to the single stranded nucleic acid, it will fluoresce orange-red at
640 nm. Therefore, acridine orange is a very informative reagent when determining the
structure of the nucleic acids that have undergone electrophoresis. Acridine orange is
also more sensitive than ethidium bromide if the electrophoresis system has been denatured
by gloxylation.
For a 1.5% agarose gel, stain with 30 mg/mL acridine orange in 10 mM sodium phosphate (pH
7.0) for 15 minutes at room temperature in the dark. To destain, run hot tap water over
the gel for 15-20 minutes.
Storage
IBI Acridine Orange should be stored at room temperature in the dark. Keep tightly sealed.
Protect from moisture.
Warning
Suspected mutagen. Avoid contact with eyes, skin, and clothing. Avoid breathing dust. Wash
thoroughly with water after handling. See Material Safety Data Sheet for additional
information. CAS # 10127-02-3.
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