10X Tris Borate EDTA Buffer
(TBE)
Catalog # Size
IB70150 1 Liter
Physical Specifications
Solution Components
0.89 M Tris
0.89 M Boric Acid
0.02 M EDTA
Conductivity (1 x solution)
850 ±100 mmhos
pH (1 x solution @ 25oC) 8.30
± 0.10
Refractive Index (10 x solution)
1.360 ±0.010
A260 (1 x solution)
<0.10 nm
A280 (1 x solution)
<0.01 nm
Molecular Biology Specifications
Gel Analysis assay Pass
Recommended Use
TBE's significantly greater buffering capacity (relative to TAE) eliminates the need for
recirculationin all but the most extended runs. This attribute makes TBE the buffer of
choice for DNA sequencing and other extended electrophoresis applications.
Storage
Store at room temperature.
Warning
Irritant. Avoid contact with eyes, skin and mucous membranes. Avoid breathing vapors. Wash
thoroughly with water after handling. See Material Safety Data Sheet for additional
information.
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